Logistical, operational and consulting services in the context of
biomedical research projects, as well as custom molecular biology laboratory services,
mutant and transgenic mouse models production, and scientific training. Available for groups
which have biological material and/or relevant clinical data but due to a lack
of available time or experience in laboratory research require support in
conceiving and designing a research project. Gene expression studies. Molecular cloning (recombinant DNA techniques).
DNA-protein interactions. Genotyping. Molecular Typing. Molecular
Biology - Proteomics.
Protein expression studies. Protein-protein interactions. Protein
separation by two-dimensional electrophoresis. Tagged protein purification
(including liquid chromatography). Production
of Mutant Animals / Genetic manipulation of the
mouse (knock-out, knock-in, deletion). CRISPR/Cas9 and homologous recombination
– mediated mutagenesis. Pronuclear Injection. ES cell Injection. Mouse Line
Rederivation. Embryo Cryopreservation. Sperm Cryopreservation. In-Vitro Fertilization.
Vasectomy and other Surgical Procedures. Bright field microscopy with image aquisition. Fluorescence Microscopy:
live cell / embryo imaging; time-lapse; ApoTome optical sectioning; confocal
microscopy. Equipment: Bio-Rad
Protean i12 IEF Cell; Bio-Rad
Trans-Blot Turbo system; Bio-Rad
Biologic LP system + BioFrac Fraction collector.
Molecular Dynamics Storm 860. MilliPore Milli-Q Integral 3. Thermo
Scientific NanoDrop 2000. Agilent
Byoanalizer 2100. Real-time PCR. Bio-Rad
T100 Thermal. Polytron PT2500E. Thermo
Scientific Savant SPD121P-230V SpeedVac concentrator. Thermo
Micron HM560. Roche
Micropipette Puller. Bio-Rad
ChemiDoc MP. Leica AM6000 automated motorized inverted microscope + TransferMan NK2
Automated Micromanipulators and Microinjectors.